Human Semaphorin 3A - SEMA3A ELISA Kit

Mfr.No.

E2078Hu

Sensitivity

0.024ng/ml

Detection Range

0.05-20ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Semaphorin 3A (also known as Sema3A) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Sema III;SEMA3A;Semaphorin III;Semaphorin-3A

Molecular Weight

88889

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E2078Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	6.5	0.46	7
Intra-Assay	2	18	1.4	0.08	5.4
Intra-Assay	3	18	2.3	0.09	4.1

Background

Involved in the development of the olfactory system and in neuronal control of puberty. Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1.

Protocol

These standard curves of E2078Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.125	2.136	2.093
	2.147
6ng/ml	1.897	1.862	1.819
	1.828
3ng/ml	1.186	1.19	1.147
	1.194
1.5ng/ml	0.626	0.673	0.63
	0.72
0.75ng/ml	0.423	0.416	0.373
	0.409
0ng/ml	0.049	0.043	0
	0.038

Gene ID	10371
UniProt ID	Q14563

Research Area	Cardiovascular

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103630	E2078Hu	48T	$320		Add to Cart
BHE12103630	E2078Hu	96T	$458		Add to Cart

Human Semaphorin 3A - SEMA3A ELISA Kit

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