Human Secretogranin 3 - SCG3 ELISA Kit

Mfr.No.

E4814Hu

Sensitivity

0.019ng/ml

Detection Range

0.05-20ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Human Secretogranin 3 (also known as Secretogranin 3) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

SCG3;Secretogranin III;Secretogranin-3;SGIII

Molecular Weight

53005

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E4814Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	8.42	0.33	3.91
Intra-Assay	2	18	5.76	0.31	5.45
Intra-Assay	3	18	2.26	0.09	3.86

Background

Member of the granin protein family that regulates the biogenesis of secretory granules (PubMed:19357184). Acts as a sorting receptor for intragranular proteins including chromogranin A/CHGA (By similarity). May also play a role in angiogenesis. Promotes endothelial proliferation, migration and tube formation through MEK/ERK signaling pathway (PubMed:29154827).

Protocol

These standard curves of E4814Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	1.727	1.819	1.763
	1.911
6ng/ml	1.099	1.102	1.046
	1.105
3ng/ml	0.595	0.592	0.536
	0.59
1.5ng/ml	0.277	0.219	0.163
	0.161
0.75ng/ml	0.059	0.069	0.013
	0.079
0ng/ml	0.061	0.056	0
	0.051

Gene ID	29106
UniProt ID	Q8WXD2

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106089	E4814Hu	48T	$320		Add to Cart
BHE12106089	E4814Hu	96T	$458		Add to Cart

Human Secretogranin 3 - SCG3 ELISA Kit

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