Human Secreted Frizzled Related Protein 3

Mfr.No.

E3897Hu

Description

This sandwich kit is for the accurate quantitative detection of human Secreted Frizzled Related Protein 3 (also known as SFRP3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Frezzled;Fritz;Frizzled-related protein 1;FRZB;FrzB-1;Secreted frizzled-related protein 3;sFRP-3

Reactivity

Human

Detection Range

0.05-30ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

36254

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.021ng/ml

Background

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP3/FRZB appears to be involved in limb skeletogenesis. Antagonist of Wnt8 signaling. Regulates chondrocyte maturation and long bone development.

Gene ID	2487
UniProt ID	Q92765
Antigen	FRZB

Research Area

Signal Transduction

Protocol

These standard curves of E3897Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
20ng/ml	2.227	2.202	2.154
	2.177
10ng/ml	1.125	1.162	1.114
	1.199
5ng/ml	0.635	0.654	0.606
	0.673
2.5ng/ml	0.493	0.494	0.446
	0.495
1.25ng/ml	0.232	0.242	0.194
	0.252
0ng/ml	0.056	0.048	0
	0.041

Precision

We measured random samples of E3897Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	5.4	0.36	6.7
Intra-Assay	2	18	7.4	0.47	6.3
Intra-Assay	3	18	13.2	0.39	3

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105335	E3897Hu	48T	$320		Add to Cart
BHE12105335	E3897Hu	96T	$458		Add to Cart

Human Secreted Frizzled Related Protein 3 - SFRP3 ELISA Kit

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