Human sE-Selectin / CD62E ELISA Kit PicoKine®

Mfr.No.

EK0501

Sensitivity

<4 pg/ml

Detection Range

125 pg/ml - 8,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	125	250	500	1000	2000	4000	8000
O.D.	0.011	0.086	0.177	0.414	0.683	1.197	1.661	2.024

Description

Human sE-Selectin / CD62E ELISA Kit PicoKine® (96 Tests). Quantitate Human SELE in cell culture supeatants, serum and plasma (heparin, citrate). Sensitivity: 4pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

E-selectin;CD62 antigen-like family member E;Endothelial leukocyte adhesion molecule 1;ELAM-1;Leukocyte-endothelial cell adhesion molecule 2;LECAM2;CD62E;SELE;ELAM1;

Molecular Weight

66655 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: W22-P556

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human sE-Selectin

Subcellular Localization

Cell membrane ; Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	152	1179	5147	138	1081	5271
Standard deviation	12	70.74	303.67	11.04	76.75	390.05
CV (%)	7.9%	6%	5.9%	8%	7.1%	7.4%

Protein Function

Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis. .

Background

E-selectin, also called endothelial-leukocyte adhesion molecule-1 (ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils. An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions. E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells (HPCs) into bone marrow (BM). A structurally and functionally related group of genes, lymph node homing receptor (LHR), granule membrane protein 140 (GMP-140), and endothelial leukocyte adhesion molecule 1 (ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1. The standard product used in this kit is recombinant human E-Selectin, excluding intercellular E-Selectin and transmembrane domain. It consists of totally 535 amino acids with the molecular mass of 58.6KDa.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	152	150	149	147	149	1.8	1.2%
Sample 2	1179	1233	1221	1130	1190	40.4	3.3%
Sample 3	5147	5002	5824	5904	5469	399.06	7.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	6401
Gene Name	SELE
UniProt ID	P16581
Protein Name	E-selectin
Sequence	Belongs to the selectin/LECAM family.

Applications	ELISA
Research Area	Signal Transduction,Tumor Suppressors,Vitamins/Minerals

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000481	EK0501	96 wells/kit	$499		Add to Cart

Human sE-Selectin / CD62E ELISA Kit PicoKine®

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