Human Sclerostin/SOST ELISA Kit PicoKine®

Mfr.No.

EK0968

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.087	0.139	0.209	0.263	0.358	0.551	0.905	1.822

Description

Human Sclerostin/SOST ELISA Kit PicoKine® (96 Tests). Quantitate Human SOST in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Sclerostin;SOST;UNQ2976/PRO7455/PRO7476;

Molecular Weight

24031 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q24-Y213

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Sclerostin

Subcellular Localization

Secreted, extracellular space, extracellular matrix .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	89	215	883	90	209	912
Standard deviation	6.75	8.6	59.16	6.74	10.65	70.22
CV (%)	6.7%	4%	6.7%	6.6%	7.7%	7.7%

Protein Function

Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation. .

Background

Sclerostin is a protein that in humans is encoded by the SOST gene. It is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Additionally, Sclerostin is produced by the osteocyte and has anti-anabolic effects on bone formation. The SOST gene maps to chromosome 17q12-q21.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	89	84	85	97	88	5.11	5.8%
Sample 2	215	183	209	209	204	12.36	6%
Sample 3	883	875	1058	953	942	73.39	7.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	50964
Gene Name	SOST
UniProt ID	Q9BQB4
Protein Name	Sclerostin
Tissue Specificity	Widely expressed at low levels with highest levels in bone, cartilage, kidney, liver, bone marrow and primary osteoblasts differentiated for 21 days. Detected in the subendothelial layer of the aortic intima (at protein level). .

Applications	ELISA
Research Area	Cytoskeleton/ECM,Developmental Biology,Extracellular Matrix,Growth Factors/Hormones,Metabolism,Organogenesis,Secreted,Signal Transduction,Signaling Pathways,Skeletal Development,Stem Cells,Structures

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001273	EK0968	96 wells/kit	$499		Add to Cart

Human Sclerostin/SOST ELISA Kit PicoKine®

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