Matrices listed below were spiked with certain level of SCG3 and the recovery rates were calculated by comparing the measured value to the expected amount of SCG3 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-100
95
EDTA plasma(n=5)
89-95
92
heparin plasma(n=5)
86-105
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SCG3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
SCG3 ELISA Kit allows for the in vitro quantitative determination of SCG3 concentrations in serum, plasma, tissue homogenates and other biological fluids.