Human Scaffold Attachment Factor B1

Mfr.No.

E3949Hu

Alternative Names

HSP27 ERE-TATA-binding protein;HSP27 estrogen response element-TATA box-binding protein;SAFB;SAF-B;SAF-B1;Scaffold attachment factor B1

Reactivity

Human

Detection Range

5-1000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

102642

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

2.56ng/L

Background

Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing (PubMed: 9671816). Functions as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription (PubMed: 12660241). Thereby acts as a negative regulator of cell proliferation (PubMed: 12660241). When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter (By similarity).

Gene ID	6294
UniProt ID	Q15424
Antigen	SAFB

Research Area

Epigenetics and Nuclear Signaling

Protocol

These standard curves of E3949Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
640ng/L	2.186	2.201	2.142
	2.215
320ng/L	1.541	1.546	1.488
	1.551
160ng/L	1.007	0.997	0.938
	0.986
80ng/L	0.635	0.634	0.576
	0.634
40ng/L	0.465	0.458	0.4
	0.451
0ng/L	0.059	0.058	0
	0.057

Precision

We measured random samples of E3949Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	400.4	22.52	5.6
Intra-Assay	2	18	63	4.38	7
Intra-Assay	3	18	141.3	8.47	6

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105383	E3949Hu	48T	$320		Add to Cart
BHE12105383	E3949Hu	96T	$458		Add to Cart

Human Scaffold Attachment Factor B1 - SAFB ELISA Kit

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