Human Sarcoplasmic Endoplasmic Reticulum Calcium Atpase 3

Mfr.No.

E6119Hu

Sensitivity

7.14ng/L

Detection Range

15-3000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

ATP2A3;Calcium pump 3;Sarcoplasmic/endoplasmic reticulum calcium ATPase 3;SERCA3;SR Ca(2+-ATPase 3

Molecular Weight

109256

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E6119Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	819.64	20.63	2.52
Intra-Assay	2	18	594.88	25.73	4.33
Intra-Assay	3	18	360.81	18.98	5.26

Background

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Transports calcium ions from the cytosol into the sarcoplasmic/endoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.

Protocol

These standard curves of E6119Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.638	2.561	2.513
	2.484
800ng/L	1.488	1.435	1.386
	1.382
400ng/L	0.732	0.788	0.74
	0.845
200ng/L	0.379	0.397	0.349
	0.415
100ng/L	0.205	0.218	0.17
	0.232
0ng/L	0.032	0.048	0
	0.064

Gene ID	489
UniProt ID	Q93084

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107481	E6119Hu	48T	$320		Add to Cart
BHE12107481	E6119Hu	96T	$458		Add to Cart

Human Sarcoplasmic Endoplasmic Reticulum Calcium Atpase 3 - ATP2A3 ELISA Kit

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