Human S100A13 ELISA Kit PicoKine®

Mfr.No.

EK1568

Sensitivity

<10 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.034	0.158	0.246	0.402	0.633	1.099	1.701	2.314

Description

Human S100A13 ELISA Kit PicoKine® (96 Tests). Quantitate Human S100A13 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Protein S100-A13;S100 calcium-binding protein A13;S100A13;

Molecular Weight

11471 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: M1-K98

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human S100A13

Subcellular Localization

Cytoplasm. Secreted. Secretion is mediated by exposure to stress and requires copper ions.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	38	138	472	38	130	466
Standard deviation	1.78	5.79	29.73	1.9	6.63	34.95
CV (%)	4.7%	4.2%	7.5%%%	5%	7.5%%	7.5%

Protein Function

Plays a role in the export of proteins that lack a signal peptide and are secreted by an alteative pathway. Binds two calcium ions per subunit. Binds one copper ion. Binding of one copper ion does not interfere with calcium binding. Required for the copper-dependent stress-induced export of IL1A and FGF1. The calcium-free protein binds to lipid vesicles containing phosphatidylserine, but not to vesicles containing phosphatidylcholine (By similarity). .

Background

S100 calcium-binding protein A13 (S100A13) is a protein that in humans is encoded by the S100A13 gene. The characterization of a human gene, S100A13 encodes a calcium-binding protein belonging to the S100 protein family. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein is widely expressed in various types of tissues with a high expression level in thyroid gland. In smooth muscle cells, this protein co-expresses with other family members in the nucleus and in stress fibers, suggesting diverse functions in signal transduction. Multiple alteatively spliced transcript variants encoding the same protein have been found for this gene.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	38	38	36	38	37	0.86	2.3%
Sample 2	138	140	138	152	142	5.83	4.1%
Sample 3	472	455	478	440	461	14.88	3.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	6284
Gene Name	S100A13
UniProt ID	Q99584
Protein Name	Protein S100-A13
Tissue Specificity	Expressed in heart and skeletal muscle.

Applications	ELISA
Research Area	Cell Biology,Cell Cycle,Cell Differentiation,Epigenetics and Nuclear Signaling,Growth Factors/Hormones,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001151	EK1568	96 wells/kit	$750		Add to Cart

Human S100A13 ELISA Kit PicoKine®

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