Human Runt-related Transcription Factor 2

Mfr.No.

E1970Hu

Sensitivity

0.1ng/ml

Detection Range

0.2-60ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Acute myeloid leukemia 3 protein;AML3;CBFA1;CBF-alpha-1;CCD;CCD1;CLCD;Core-binding factor subunit alpha-1;Oncogene AML-3;OSF2;OSF-2;Osteoblast-specific transcription factor 2;PEA2aA;PEA2-alpha A;PEBP2aA;PEBP2-alpha A;Polyomavirus enhancer-binding protein 2 alpha A subunit;Runt-related transcription factor 2;RUNX2;SL3/AKV core-binding factor alpha A subunit;SL3-3 enhancer factor 1 alpha A subunit

Molecular Weight

56648

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E1970Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	7.98	0.43	5.35
Intra-Assay	2	18	14.22	0.5	3.52
Intra-Assay	3	18	5.67	0.36	6.29

Background

Runt-related transcription factor 2 (RUNX2) also known as core-binding factor subunit alpha-1 (CBF-alpha-1) is a protein that in humans is encoded by the RUNX2 gene. RUNX2 is a key transcription factor associated with osteoblast differentiation.

Protocol

These standard curves of E1970Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
32ng/ml	2.258	2.189	2.155
	2.121
16ng/ml	1.517	1.528	1.494
	1.54
8ng/ml	0.834	0.821	0.787
	0.808
4ng/ml	0.456	0.461	0.427
	0.465
2ng/ml	0.293	0.255	0.221
	0.218
0ng/ml	0.034	0.034	0
	0.034

Gene ID	860
UniProt ID	Q13950

Research Area	Neuroscience

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103532	E1970Hu	48T	$320		Add to Cart
BHE12103532	E1970Hu	96T	$458		Add to Cart

Human Runt-related Transcription Factor 2 - RUNX2 ELISA Kit

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