Human Rhophilin 2 - RHPN2 ELISA Kit

Mfr.No.

E2512Hu

Alternative Names

76 kDa RhoB effector protein;GTP-Rho-binding protein 2;p76RBE;Rhophilin-2;RHPN 2;RHPN2

Reactivity

Human

Detection Range

15-3000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

76993

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

8.17ng/L

Background

Binds specifically to GTP-Rho. May function in a Rho pathway to limit stress fiber formation and/or increase the turnover of F-actin structures in the absence of high levels of RhoA activity.

Gene ID	85415
UniProt ID	Q8IUC4
Antigen	RHPN2

Research Area

Others

Protocol

These standard curves of E2512Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	1.826	1.782	1.742
	1.737
800ng/L	0.994	1.029	0.989
	1.064
400ng/L	0.64	0.639	0.599
	0.639
200ng/L	0.219	0.241	0.201
	0.264
100ng/L	0.146	0.133	0.093
	0.121
0ng/L	0.045	0.04	0
	0.035

Precision

We measured random samples of E2512Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	373.7	20.42	5.5
Intra-Assay	2	18	535.4	23.88	4.5
Intra-Assay	3	18	1512.4	68.15	4.5

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104050	E2512Hu	48T	$320		Add to Cart
BHE12104050	E2512Hu	96T	$458		Add to Cart

Human Rhophilin 2 - RHPN2 ELISA Kit

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