Human Rho Guanine Nucleotide Exchange Factor 5

Mfr.No.

E3238Hu

Alternative Names

ARHGEF5;Ephexin-3;GEF5;Guanine nucleotide regulatory protein TIM;Oncogene TIM;P60;p60 TIM;Rho guanine nucleotide exchange factor 5;TIM;TIM1;Transforming immortalized mammary oncogene

Reactivity

Human

Detection Range

7-1500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

176799

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

3.98ng/L

Background

Rho guanine nucleotide exchange factor 5 is a protein that in humans is encoded by the ARHGEF5 gene. Rho GTPases play a fundamental role in numerous cellular processes initiated by extracellular stimuli that work through G protein-coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein may be involved in the control of cytoskeletal organization.

Gene ID	7984
UniProt ID	Q12774
Antigen	ARHGEF5

Research Area

Signal Transduction

Protocol

These standard curves of E3238Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800ng/L	1.737	1.84	1.785
	1.943
400ng/L	0.92	0.963	0.908
	1.005
200ng/L	0.537	0.532	0.478
	0.527
100ng/L	0.156	0.219	0.164
	0.282
50ng/L	0.089	0.082	0.028
	0.076
0ng/L	0.052	0.055	0
	0.058

Precision

We measured random samples of E3238Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	225.84	10.28	4.55
Intra-Assay	2	18	473.53	21.59	4.56
Intra-Assay	3	18	572.57	14.1	2.46

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104767	E3238Hu	48T	$320		Add to Cart
BHE12104767	E3238Hu	96T	$458		Add to Cart

Human Rho Guanine Nucleotide Exchange Factor 5 - ARHGEF5 ELISA Kit

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