Human Retinol Binding Protein 4

Mfr.No.

E1206Hu

Sensitivity

0.51ng/ml

Detection Range

1-400ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Retinol Binding Protein 4 (also known as RBP-4) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Plasma retinol-binding protein;Plasma retinol-binding protein(1-176;Plasma retinol-binding protein(1-179;Plasma retinol-binding protein(1-181;Plasma retinol-binding protein(1-182;PRBP;RBP;RBP 4;RBP4;Retinol-binding protein 4

Molecular Weight

23010

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E1206Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	55.5	3.32	6
Intra-Assay	2	18	95.8	4.39	4.6
Intra-Assay	3	18	124.7	5.51	4.4

Background

Retinol-binding protein that mediates retinol transport in blood plasma (PubMed: 5541771). Delivers retinol from the liver stores to the peripheral tissues (Probable). Transfers the bound all-trans retinol to STRA6, that then facilitates retinol transport across the cell membrane (PubMed: 22665496).

Protocol

These standard curves of E1206Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
240ng/ml	2.13	2.118	2.054
	2.107
120ng/ml	1.279	1.334	1.269
	1.389
60ng/ml	0.869	0.803	0.739
	0.737
30ng/ml	0.478	0.476	0.412
	0.475
15ng/ml	0.275	0.298	0.233
	0.321
0ng/ml	0.057	0.065	0
	0.072

Gene ID	5950
UniProt ID	P02753

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102852	E1206Hu	48T	$320		Add to Cart
BHE12102852	E1206Hu	96T	$458		Add to Cart

Human Retinol Binding Protein 4 - RBP-4 ELISA Kit

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