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Home / ELISA Kit

Human Retinol-binding protein 3 (RBP3) ELISA Kit

SKU : BHE13703035

Retinol-binding protein 3, interstitial (RBP3), also known as IRBP is a protein identified in most eutherians except tenrecs and armadillos.The interphotoreceptor retinol-binding protein is a large glycoprotein known to bind retinoids and found primarily in the interphotoreceptor matrix of the retina between the retinal pigment epithelium and the photoreceptor cells. It is thought to transport retinoids between the retinal pigment epithelium and the photoreceptors, a critical role in the visual process. The human IRBP gene is approximately 9.5 kbp in length and consists of four exons separated by three introns. The introns are 1.6-1.9 kbp long. The gene is transcribed by photoreceptor and retinoblastoma cells into an approximately 4.3-kilobase mRNA that is translated and processed into a glycosylated protein of 135,000 Da.

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Mfr.No.	KTE60819
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Retinol-binding protein 3 (RBP3) ELISA Kit employs a two-site sandwich ELISA to quantitate RBP3.
Alternative Names	RBP3; D10S64; D10S65; D10S66; IRBP; RBPI; interphotoreceptor retinoid-binding protein; retinol-binding protein 3; retinol-binding protein 3; interstitial
Detection Method	Colorimetric
Kit Components	Human Retinol-binding protein 3 microplate Human Retinol-binding protein 3 standard Human Retinol-binding protein 3 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Retinol-binding protein 3 (RBP3) ELISA Kit has high sensitivity and excellent specificity for detection of Human RBP3. No significant cross-reactivity or interference between Human RBP3 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Retinol-binding protein 3, interstitial (RBP3), also known as IRBP is a protein identified in most eutherians except tenrecs and armadillos.The interphotoreceptor retinol-binding protein is a large glycoprotein known to bind retinoids and found primarily in the interphotoreceptor matrix of the retina between the retinal pigment epithelium and the photoreceptor cells. It is thought to transport retinoids between the retinal pigment epithelium and the photoreceptors, a critical role in the visual process. The human IRBP gene is approximately 9.5 kbp in length and consists of four exons separated by three introns. The introns are 1.6-1.9 kbp long. The gene is transcribed by photoreceptor and retinoblastoma cells into an approximately 4.3-kilobase mRNA that is translated and processed into a glycosylated protein of 135,000 Da.
Notes	This Human Retinol-binding protein 3 (RBP3) ELISA Kit employs a two-site sandwich ELISA to quantitate RBP3 in samples. An antibody specific for RBP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRBP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RBP3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RBP3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	5949

Applications	ELISA
Applications Notes	This Human Retinol-binding protein 3 (RBP3) ELISA Kit employs a two-site sandwich ELISA to quantitate RBP3 in samples. An antibody specific for RBP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRBP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RBP3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RBP3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703035	KTE60819	48 T	$339		Add to Cart
BHE13703035	KTE60819	96 T	$589		Add to Cart
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