Human RET ELISA Kit PicoKine®

Mfr.No.

EK1877

Sensitivity

<15 pg/ml

Detection Range

93.8 pg/ml - 6,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	93.8	187.5	375	750	1500	3000	6000
O.D.	0.002	0.068	0.123	0.245	0.485	0.907	1.482	2.050

Description

Human RET ELISA Kit PicoKine® (96 Tests). Quantitate Human RET in cell culture supeatants, serum and plasma (heparin). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Proto-oncogene tyrosine-protein kinase receptor Ret; Cadherin family member 12; Proto-oncogene c-Ret; RET; CDHF12; CDHR16; PTC; RET51

Immunogen

Expression system for standard: NS0; Immunogen sequence: L29-R635

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human RET

Subcellular Localization

Endosome membrane, Single-pass type I membrane protein, Cell membrane,

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	148	628	2813	162	691	2933
Standard deviation	6.96	32.03	149.09	9.72	44.92	231.71
CV (%)	4.7%	5.1%	5.3%	6.0%	6.5%	7.9%

Protein Function

Receptor tyrosine-protein kinase involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation upon binding with glial cell derived neurotrophic factor family ligands. Phosphorylates P

Background

This gene encodes a transmembrane receptor and member of the tyrosine protein kinase family of proteins. It is mapped to 10q11.21. Binding of ligands such as GDNF (glial cell-line derived neurotrophic factor) and other related proteins to the encoded receptor stimulates receptor dimerization and activation of downstream signaling pathways that play a role in cell differentiation, growth, migration and survival. The encoded receptor is important in development of the nervous system, and the development of organs and tissues derived from the neural crest. This proto-oncogene can undergo oncogenic activation through both cytogenetic rearrangement and activating point mutations. Mutations in this gene are associated with Hirschsprung disease and central hypoventilation syndrome and have been identified in patients with renal agenesis.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	148	147	153	148	149	2.34	1.5%
Sample 2	628	656	611	669	641	22.79	3.5%
Sample 3	2813	3203	3280	2837	3033	210.19	6.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	5979
Gene Name	RET
UniProt ID	P07949

Applications	ELISA
Research Area	Cancer,Oncoproteins,Oncoproteins/Suppressors,Protein Phosphorylation,Receptor Tyrosine Kinases,Signal Transduction,Tyrosine Kinases

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001425	EK1877	96 wells/kit	$499		Add to Cart

Human RET ELISA Kit PicoKine®

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