Human Restin - RSN ELISA Kit

Mfr.No.

E2936Hu

Alternative Names

NES;Nestin

Reactivity

Human

Detection Range

0.1-40ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

177439

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.046ng/ml

Background

Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity).

Gene ID	10763
UniProt ID	P48681
Antigen	NES

Research Area

Others

Protocol

These standard curves of E2936Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/ml	2.098	2.101	2.051
	2.103
12ng/ml	1.069	1.11	1.06
	1.151
6ng/ml	0.539	0.506	0.456
	0.473
3ng/ml	0.262	0.281	0.231
	0.301
1.5ng/ml	0.166	0.153	0.104
	0.141
0ng/ml	0.059	0.05	0
	0.04

Precision

We measured random samples of E2936Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	8.2	0.36	4.4
Intra-Assay	2	18	8.3	0.3	3.6
Intra-Assay	3	18	19.5	0.63	3.2

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104473	E2936Hu	48T	$320		Add to Cart
BHE12104473	E2936Hu	96T	$458		Add to Cart

Human Restin - RSN ELISA Kit

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