Human Resistin - RETN ELISA Kit

Mfr.No.

E0338Hu

Description

This sandwich kit is for the accurate quantitative detection of Resistin (also known as Retn) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Adipose tissue-specific secretory factor;ADSF;C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein;Cysteine-rich secreted protein A12-alpha-like 2;Cysteine-rich secreted protein FIZZ3;RESISTIN

Reactivity

Human

Detection Range

20-6000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

11419

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

10.2ng/L

Background

Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells (By similarity). Potentially links obesity to diabetes (By similarity). Promotes chemotaxis in myeloid cells (PubMed: 15064728).

Gene ID	56729
UniProt ID	Q9HD89
Antigen	RESISTIN

Research Area

Cardiovascular

Protocol

These standard curves of E0338Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
3200ng/L	2.245	2.256	2.232
	2.268
1600ng/L	1.396	1.391	1.366
	1.385
800ng/L	0.587	0.618	0.594
	0.65
400ng/L	0.42	0.386	0.361
	0.352
200ng/L	0.214	0.176	0.152
	0.138
0ng/L	0.016	0.025	0
	0.034

Precision

We measured random samples of E0338Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	649.4	28.97	4.5
Intra-Assay	2	18	1217.6	59.41	4.9
Intra-Assay	3	18	1594.3	58.35	3.7

Storage	2-8°C

Reference	Adipocytokines and the risk of coronary heart disease in healthy middle aged men: the PRIME Study Journal: Int J Obes (Lond). 2010 Jan;34(1):118-26. Significance of Some Non-Invasive Biomarkers in the Early Diagnosis and Staging of Egyptian Breast Cancer Patients Journal: Asian Pac J Cancer Prev. 2020 Nov 1;21(11):3279-3284. Increased Serum Levels of Tumor Necrosis Factor-Alpha, Resistin, and Visfatin in the Children with Autism Spectrum Disorders: A Case-Control Study Journal: Neurol Res Int. 2016;2016:9060751. Oral ?-lipoic acid supplementation in patients with non-alcoholic fatty liver disease: effects on adipokines and liver histology features Journal: Food Funct. 2019 Aug 1;10(8):4941-4952.

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102041	E0338Hu	96T	$458		Add to Cart
BHE12102041	E0338Hu	48T	$320		Add to Cart

Human Resistin - RETN ELISA Kit

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