Human Resistin-like Molecule beta (RELM-beta) ELISA Kit

Mfr.No.

CSB-E13852h

Sensitivity

11.7 pg/mL

Detection Range

47 pg/mL-3000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

resistin like beta, FIZZ1, FIZZ2, HXCP2, RELM-beta, RELMb, RELMbeta, XCP2, C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 2, colon and small intestine-specific cysteine-rich protein, cy

Recovery

The recovery of human CKM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	89	85-93

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CKM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	84
	Range %	80-88
1:200	Average %	98
	Range %	94-102
1:400	Average %	90
	Range %	86-94
1:800	Average %	106
	Range %	101-113

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

?U/ml	OD1	OD2	Average
200	0.155	0.161	0.158
100	0.272	0.260	0.266
50	0.443	0.455	0.449
25	0.706	0.682	0.694
12.5	1.045	1.007	1.026
6.25	1.248	1.293	1.271
3.12	1.642	1.714	1.678
0	2.055	2.159	2.107

Species

Homo sapiens

Sample Type

Serum, Cell culture supernatants, Plasma

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	Q9BQ08
Tissue Specificity	Expressed only in the gastrointestinal tract, particularly the colon.

Applications	ELISA
Research Area	RETNLB

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504144	CSB-E13852h	96T	$790	Add to Cart
BHE10504144	CSB-E13852h	5x96T	$3792	Add to Cart
BHE10504144	CSB-E13852h	10x96T	$7505	Add to Cart

Human Resistin-like Molecule beta (RELM-beta) ELISA Kit

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