Human Resistin ELISA Kit PicoKine® (96 Tests). Quantitate Human RETN in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 3pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Alternative Names
Resistin;Adipose tissue-specific secretory factor;ADSF;C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein;Cysteine-rich secreted protein A12-alpha-like 2;Cysteine-rich secreted protein FIZZ3;RETN;FIZZ3, HXCP1, RSTN;UNQ407/PRO1199;
Molecular Weight
11419 MW
Immunogen
Expression system for standard: E.coli; Immunogen sequence: K19-P108
Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes.
Background
Resistin is an adipose-derived hormone postulated to link adiposity to insulin resistance.1 Type 2 diabetes, characterized by target-tissue resistance to insulin, is epidemic in industrialized societies and is strongly associated with obesity. Adipocytes secrete a unique signalling molecule, which called resistin (for resistance to insulin), that may be the hormone potentially links obesity to diabetes.2 Resistin is overexpressed in human adipose tissue of obese individuals and is likely to modulate insulin sensitivity. Resistin is, therefore, a candidate gene for insulin resistance.3 The standard product used in this kit is human Resistin with the molecular mass of 51-53KDa. The detected Resistin includes zymogen and active enzyme.
Reproducibility
Lots
Lot 1 (pg/ml)
Lot 2 (pg/ml)
Lot 3 (pg/ml)
Lot 4 (pg/ml)
Mean (pg/ml)
Standard Deviation
CV (%)
Sample 1
135
137
143
146
140
4.43
3.1%
Sample 2
639
653
634
737
665
41.72
6.2%
Sample 3
2067
2270
2337
2337
2252
110.67
4.9%
*number of samples for each test n=16.
Notes
Material Required But Not Provided: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer . Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Gene ID
56729
Gene Name
RETN
UniProt ID
Q9HD89
Protein Name
Resistin
Tissue Specificity
Expressed only in fatty tissues.
Sequence
Belongs to the resistin/FIZZ family.
Applications
ELISA
Research Area
Atherosclerosis,Cardiovascular,Diabetes-associated,Energy Metabolism,Energy Transfer Pathways,Growth Factors/Hormones,Metabolic Signaling Pathways,Metabolism,Pathways and Processes,Signal Transduction
Storage
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)