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Home / ELISA Kit

Human Replication initiator 1 (REPIN1) ELISA Kit

SKU : BHE13703022

Replication initiator 1 is a protein encoded by the REPIN1 gene. Homodimers of RIP60 (replication initiation-region protein 60 kDA) purified from nuclear extract bind two ATT-rich sites in oribeta and foster the formation of a twisted 720 bp DNA loop in vitro.
RIP60 contains 15 C(2)H(2)zinc finger (ZF) DNA binding motifs organized in three clusters, termed hand Z1 (ZFs 1-5), hand Z2 (ZFs 6-8) and hand Z3 (ZFs 9-15). A proline-rich region is located between hands Z2 and Z3. Gel mobility shift and DNase I footprinting experiments show hands Z1 and Z2 independently bind the oribeta RIP60 sites specifically, but with different affinities. Hand Z3 binds DNA, but displays no specificity for RIP60 sites.

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Mfr.No.	KTE60806
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Replication initiator 1 (REPIN1) ELISA Kit employs a two-site sandwich ELISA to quantitate REPIN1.
Alternative Names	REPIN1; AP4; RIP60; ZNF464; Zfp464; H_DJ0584D14.12; replication initiation region protein (60kD); zinc finger protein 464 (RIP60); zinc finger protein AP4; zinc finger proten AP4
Detection Method	Colorimetric
Kit Components	Human Replication initiator 1 microplate Human Replication initiator 1 standard Human Replication initiator 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Replication initiator 1 (REPIN1) ELISA Kit has high sensitivity and excellent specificity for detection of Human REPIN1. No significant cross-reactivity or interference between Human REPIN1 and analogues was observed.
Calibration Range	Please inquire
Sample Type	Serum, Cell culture supernatants, Plasma, Other biological fluids
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Replication initiator 1 is a protein encoded by the REPIN1 gene. Homodimers of RIP60 (replication initiation-region protein 60 kDA) purified from nuclear extract bind two ATT-rich sites in oribeta and foster the formation of a twisted 720 bp DNA loop in vitro. RIP60 contains 15 C(2)H(2)zinc finger (ZF) DNA binding motifs organized in three clusters, termed hand Z1 (ZFs 1-5), hand Z2 (ZFs 6-8) and hand Z3 (ZFs 9-15). A proline-rich region is located between hands Z2 and Z3. Gel mobility shift and DNase I footprinting experiments show hands Z1 and Z2 independently bind the oribeta RIP60 sites specifically, but with different affinities. Hand Z3 binds DNA, but displays no specificity for RIP60 sites.
Notes	This Human Replication initiator 1 (REPIN1) ELISA Kit employs a two-site sandwich ELISA to quantitate REPIN1 in samples. An antibody specific for REPIN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyREPIN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for REPIN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of REPIN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	29803

Applications	ELISA
Applications Notes	This Human Replication initiator 1 (REPIN1) ELISA Kit employs a two-site sandwich ELISA to quantitate REPIN1 in samples. An antibody specific for REPIN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyREPIN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for REPIN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of REPIN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703022	KTE60806	48 T	$339		Add to Cart
BHE13703022	KTE60806	96 T	$589		Add to Cart
BHE13703022	KTE60806	96 Tx5	$2629		Add to Cart
BHE13703022	KTE60806	96 Tx50	$24569		Add to Cart

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