Human Replication Initiator 1

Mfr.No.

E2401Hu

Alternative Names

60 kDa origin-specific DNA-binding protein;60 kDa replication initiation region protein;ATT-binding protein;DHFR oribeta-binding protein RIP60;REPIN 1;REPIN1;Replication initiator 1;Zinc finger protein 464

Reactivity

Human

Detection Range

20-4500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

63575

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

10.48ng/L

Background

Sequence-specific double-stranded DNA-binding protein required for initiation of chromosomal DNA replication. Binds on 5'-ATT-3' reiterated sequences downstream of the origin of bidirectional replication (OBR) and a second, homologous ATT sequence of opposite orientation situated within the OBR zone. Facilitates DNA bending.

Gene ID	29803
UniProt ID	Q9BWE0
Antigen	REPIN1

Research Area

Others

Protocol

These standard curves of E2401Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	1.995	1.985	1.946
	1.975
1200ng/L	1.112	0.997	0.958
	0.882
600ng/L	0.604	0.587	0.547
	0.569
300ng/L	0.155	0.166	0.127
	0.176
150ng/L	0.08	0.108	0.069
	0.136
0ng/L	0.027	0.039	0
	0.051

Precision

We measured random samples of E2401Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1649.9	48.98	3
Intra-Assay	2	18	515.9	20.09	3.9
Intra-Assay	3	18	902.6	32.9	3.6

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103939	E2401Hu	48T	$320		Add to Cart
BHE12103939	E2401Hu	96T	$458		Add to Cart

Human Replication Initiator 1 - REPIN1 ELISA Kit

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