Human Renin ELISA Kit

Mfr.No.

E1016Hu

Description

This sandwich kit is for the accurate quantitative detection of Human Renin (also known as RENIN) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Angiotensinogenase;REN;Renin

Reactivity

Human

Detection Range

3-900ng/L

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

45057

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

1.63ng/L

Background

Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.

Gene ID	5972
UniProt ID	P00797
Antigen	REN

Research Area

Cardiovascular

Protocol

These standard curves of E1016Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
480ng/L	2.248	2.222	2.177
	2.195
240ng/L	1.474	1.461	1.416
	1.447
120ng/L	0.899	0.877	0.833
	0.854
60ng/L	0.498	0.485	0.44
	0.471
30ng/L	0.314	0.271	0.227
	0.229
0ng/L	0.046	0.044	0
	0.043

Precision

We measured random samples of E1016Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	119.1	8.04	6.8
Intra-Assay	2	18	200.9	7.78	3.9
Intra-Assay	3	18	100	6.27	6.3

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102680	E1016Hu	48T	$320		Add to Cart
BHE12102680	E1016Hu	96T	$458		Add to Cart

Human Renin ELISA Kit

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