Human Relaxin 1 ELISA Kit PicoKine®

Mfr.No.

EK1230

Sensitivity

<10 pg/ml

Detection Range

78 pg/ml - 5,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	78	156	312	625	1250	2500	5000
O.D.	0.033	0.040	0.052	0.093	0.172	0.409	1.028	2.108

Description

Human Relaxin 1 ELISA Kit PicoKine® (96 Tests). Quantitate Human RLN1 in cell culture supeatants, serum and plasma (heparin). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Prorelaxin H1;Relaxin B chain;Relaxin A chain;RLN1;

Molecular Weight

21146 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: R162-Cys185 (A) & K26-S53 (B)

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Relaxin 1

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	144	819	2390	133	796	2573
Standard deviation	10.94	37.67	100.38	10.5	42.98	118.35
CV (%)	7.6%	4.6%	4.2%	7.9%	5.4%	4.6%

Protein Function

Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals. May be involved in remodeling of connective tissues during pregnancy, promoting growth of pubic ligaments and ripening of the cervix.

Background

Relaxin 1 is a member of relaxin-like peptide family. Relaxin gene maps to human chromosome 19 near D19Mit23. Relaxin is a peptide hormone produced by the corpora lutea of ovaries during pregnancy in many mammalian species, including man. Relaxin widens the pubic bone and facilitates labor, it also softens the cervix (cervical ripening), and relaxes the uterine musculature. However, its significance may reach much further. Relaxin affects collagen metabolism, inhibiting collagen synthesis and enhancing its breakdown by increasing matrix metalloproteinases. It also enhances angiogenesis and is a potent renal vasodilator.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	144	125	144	133	136	8.01	5.8%
Sample 2	819	759	734	688	750	47.28	6.3%
Sample 3	2390	2222	2307	2378	2324	67.01	2.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	6013
Gene Name	RLN1
UniProt ID	P04808
Protein Name	Prorelaxin 1
Tissue Specificity	Prostate. Not expressed in placenta, decidua or ovary. .

Applications	ELISA
Research Area	Cell Type Markers,Growth Factors/Hormones,Signal Transduction,Tags & Cell Markers

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000988	EK1230	96 wells/kit	$499		Add to Cart

Human Relaxin 1 ELISA Kit PicoKine®

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