Human REG3A ELISA Kit PicoKine®

Mfr.No.

EK1834

Sensitivity

<0.1 ng/ml

Detection Range

0.78 ng/ml - 50 ng/ml

Reactivity

Human

Sample Volume

Concentration (ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.022	0.151	0.243	0.470	0.820	1.267	1.765	2.075

Description

Human REG3A ELISA Kit PicoKine® (96 Tests). Quantitate Human REG3A in cell culture supeatants, serum and plasma (heparin). Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Regenerating islet-derived protein 3-alpha; REG-3-alpha; Hepatointestinal pancreatic protein; HIP; PAP; Human proislet peptide; Pancreatitis-associated protein 1; Regenerating islet-derived protein III-alpha; Reg III-alpha; REG3A; PAP1

Immunogen

Expression system for standard: NS0; Immunogen sequence: E27-D175

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human REG3A

Subcellular Localization

Secreted

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	1176	6316	19450	1433	6401	19872
Standard deviation	58.80	385.28	836.35	65.92	409.66	1430.78
CV (%)	5.0%	6.1%	4.3%	4.6%	6.4%	7.2%

Protein Function

Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Regulates keratinocyte proliferation and differentiation after skin injur

Background

Regenerating islet-derived protein 3 alpha (or Regenerating islet-derived protein III-alpha) formerly known as HIP/PAP (Hepatocarcinoma-Intestine-Pancreas/Pancreatitis-Associated Protein) and peptide 23 is a protein that in humans is encoded by the REG3A gene. This gene is mapped to 2p12. It encodes a pancreatic secretory protein that may be involved in cell proliferation or differentiation. It has similarity to the C-type lectin superfamily. The enhanced expression of this gene is observed during pancreatic inflammation and liver carcinogenesis. The mature protein also functions as an antimicrobial protein with antibacterial activity. Alteate splicing results in multiple transcript variants that encode the same protein.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	1176	1259	1226	1140	1200	45.64	3.8%
Sample 2	6316	7061	7332	7319	7007	413.32	5.8%
Sample 3	19450	23051	22840	23122	22115	1542.56	6.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	5068
Gene Name	REG3A
UniProt ID	Q06141
Tissue Specificity	Highly expressed in epidermal keratinocytes of psoriasis patients (at protein level). Constitutively expressed in intestine. Low expression is found in healthy pancreas. Overexpressed during the acute phase of pancreatitis and in some patients with chroni

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001411	EK1834	96 wells/kit	$499		Add to Cart

Human REG3A ELISA Kit PicoKine®

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