Human Receptor-type Tyrosine-protein Phosphatase F

Mfr.No.

E6068Hu

Sensitivity

0.83ng/L

Detection Range

2-700ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

BNAH2;LAR;LARS;Leukocyte common antigen related;PTPRF;Receptor-type tyrosine-protein phosphatase F

Molecular Weight

212879

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E6068Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	190.11	8.21	4.32
Intra-Assay	2	18	337.43	9.48	2.81
Intra-Assay	3	18	77.85	3.5	4.5

Background

Possible cell adhesion receptor. It possesses an intrinsic protein tyrosine phosphatase activity (PTPase) and dephosphorylates EPHA2 regulating its activity. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one.

Protocol

These standard curves of E6068Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
400ng/L	1.996	1.968	1.921
	1.939
200ng/L	1.115	1.011	0.964
	0.907
100ng/L	0.535	0.549	0.503
	0.563
50ng/L	0.28	0.216	0.17
	0.153
25ng/L	0.105	0.121	0.075
	0.138
0ng/L	0.038	0.046	0
	0.054

Gene ID	5792
UniProt ID	P10586

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107430	E6068Hu	48T	$320		Add to Cart
BHE12107430	E6068Hu	96T	$458		Add to Cart

Human Receptor-type Tyrosine-protein Phosphatase F - PTPRF ELISA Kit

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