Human Rap Gguanine Nucleotide Exchange Factor 4

Mfr.No.

E6056Hu

Sensitivity

7.67ng/L

Detection Range

15-3000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

CAMP-GEFII;cAMP-regulated guanine nucleotide exchange factor II;CGEF2;EPAC;EPAC 2;EPAC2;Exchange factor directly activated by cAMP 2;Exchange protein directly activated by cAMP 2;Nbla00496;Rap guanine nucleotide exchange factor 4;RAPGEF4

Molecular Weight

115522

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E6056Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	507.52	25.36	5
Intra-Assay	2	18	871.86	29.03	3.33
Intra-Assay	3	18	178.83	9.66	5.4

Background

Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2 (By similarity).

Protocol

These standard curves of E6056Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.235	2.134	2.064
	2.033
800ng/L	1.425	1.416	1.346
	1.407
400ng/L	0.827	0.839	0.769
	0.851
200ng/L	0.504	0.458	0.388
	0.412
100ng/L	0.272	0.294	0.223
	0.316
0ng/L	0.071	0.07	0
	0.069

Gene ID	11069
UniProt ID	Q8WZA2

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107418	E6056Hu	48T	$320		Add to Cart
BHE12107418	E6056Hu	96T	$458		Add to Cart

Human Rap Gguanine Nucleotide Exchange Factor 4 - RAPGEF4 ELISA Kit

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