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Home / ELISA Kit

Human Putative platelet basic protein-like 2 (PPBPL2) ELISA Kit

SKU : BHE13703409

By screening an adult human lung cDNA library, Luzi and Strayer (1995) identified PPBPL2, which they called A2. The deduced protein contains 106 amino acids. By gel retardation assay, Luzi and Strayer (1995) demonstrated that PPBPL2 exhibited binding activity to a 212-bp region of the SPB (SFTPB) promoter and slightly increased transcription of a luciferase reporter containing the promoter region.
Beta-thromboglobulin (beta-thromboglobulin, also called Pro-Platelet basic protein) is a protein that is stored in alpha-granules of platelets and released in large amounts after platelet activation.It is a chemoattractant, strongly for fibroblasts and weakly for neutrophils. It is a stimulator of mitogenesis, extracellular matrix synthesis, glucose metabolism, and plasminogen activator synthesis in human fibroblasts.

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Mfr.No.	KTE61193
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Putative platelet basic protein-like 2 (PPBPL2) ELISA Kit employs a two-site sandwich ELISA to quantitate PPBPL2.
Alternative Names	PPBPL2; SPBPBP;
Detection Method	Colorimetric
Kit Components	Human Putative platelet basic protein-like 2 microplate Human Putative platelet basic protein-like 2 standard Human Putative platelet basic protein-like 2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Putative platelet basic protein-like 2 (PPBPL2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PPBPL2. No significant cross-reactivity or interference between Human PPBPL2 and analogues was observed.
Calibration Range	Please inquire
Sample Type	Serum, Cell culture supeatants, Plasma, Other biological fluids
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	By screening an adult human lung cDNA library, Luzi and Strayer (1995) identified PPBPL2, which they called A2. The deduced protein contains 106 amino acids. By gel retardation assay, Luzi and Strayer (1995) demonstrated that PPBPL2 exhibited binding activity to a 212-bp region of the SPB (SFTPB) promoter and slightly increased transcription of a luciferase reporter containing the promoter region. Beta-thromboglobulin (beta-thromboglobulin, also called Pro-Platelet basic protein) is a protein that is stored in alpha-granules of platelets and released in large amounts after platelet activation.It is a chemoattractant, strongly for fibroblasts and weakly for neutrophils. It is a stimulator of mitogenesis, extracellular matrix synthesis, glucose metabolism, and plasminogen activator synthesis in human fibroblasts.
Notes	This Human Putative platelet basic protein-like 2 (PPBPL2) ELISA Kit employs a two-site sandwich ELISA to quantitate PPBPL2 in samples. An antibody specific for PPBPL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPBPL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPBPL2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPBPL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Putative platelet basic protein-like 2 (PPBPL2) ELISA Kit employs a two-site sandwich ELISA to quantitate PPBPL2 in samples. An antibody specific for PPBPL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPBPL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPBPL2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPBPL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703409	KTE61193	48 T	$339		Add to Cart
BHE13703409	KTE61193	96 T	$589		Add to Cart
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BHE13703409	KTE61193	96 Tx50	$24569		Add to Cart

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