Human Purine Nucleoside Phosphorylase

Mfr.No.

E3384Hu

Alternative Names

Inosine phosphorylase;Inosine-guanosine phosphorylase;PNP;Purine nucleoside phosphorylase

Reactivity

Human

Detection Range

3-900ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

32118

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

1.32ng/L

Background

Catalyzes the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate (PubMed: 9305964, PubMed: 23438750). Preferentially acts on 6-oxopurine nucleosides including inosine and guanosine (PubMed: 9305964).

Gene ID	4860
UniProt ID	P00491
Antigen	PNP

Research Area

Neuroscience

Protocol

These standard curves of E3384Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
480ng/L	2.07	2.123	2.074
	2.177
240ng/L	1.964	1.921	1.871
	1.878
120ng/L	1.082	1.135	1.086
	1.189
60ng/L	0.716	0.713	0.663
	0.71
30ng/L	0.439	0.439	0.39
	0.439
0ng/L	0.061	0.05	0
	0.038

Precision

We measured random samples of E3384Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	138.3	6.12	4.4
Intra-Assay	2	18	69.8	2.95	4.2
Intra-Assay	3	18	38.5	2.17	5.6

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104912	E3384Hu	48T	$320		Add to Cart
BHE12104912	E3384Hu	96T	$458		Add to Cart

Human Purine Nucleoside Phosphorylase - PNP ELISA Kit

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