Human PTX3/Pentraxin 3 ELISA Kit PicoKine®

Mfr.No.

EK0861

Sensitivity

<10 pg/ml

Detection Range

312 pg/ml - 20,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.052	0.150	0.278	0.420	0.649	1.111	1.605	1.941

Description

Human PTX3/Pentraxin 3 ELISA Kit PicoKine® (96 Tests). Quantitate Human PTX3 in cell culture supeatants, serum, plasma (EDTA) and saliva. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Pentraxin-related protein PTX3;Pentaxin-related protein PTX3;Tumor necrosis factor alpha-induced protein 5;TNF alpha-induced protein 5;Tumor necrosis factor-inducible gene 14 protein;TSG-14;PTX3;TNFAIP5, TSG14;

Molecular Weight

41976 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: E18-S381

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human PTX3

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma, Saliva

Anticoagulant

EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	662	1701	9194	718	1816	9601
Standard deviation	33.76	85.05	579.22	38.77	92.61	662.46
CV (%)	6.9%	5%	6.9%	5.4%	6.9%	6.9%

Protein Function

Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self- components and female fertility. .

Background

PTX3 (Pentraxin 3) is a member of the pentraxin superfamily. This super family characterized by cyclic multimeric structure. The PTX3 gene is mapped to 3q25.32. The predicted 381-amino acid PTX3 protein has homology to the pentraxin protein family. Significant levels of PTX3 were detected in plasma of neutropenic patients with systemic A. PTX3 is effective in preventing CMV infection and reactivation, as well as subsequent Aspergillus infection. PTX3 activates the classical pathway of complementactivation and facilitates pathogen recognition by macrophages and DCs.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	662	620	693	604	644	34.99	5.4%
Sample 2	1701	1556	1536	1612	1601	63.97	3.9%
Sample 3	9194	8999	8940	8763	8974	153.86	1.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	5806
Gene Name	PTX3
UniProt ID	P26022
Protein Name	Pentraxin-related protein PTX3

Applications	ELISA
Research Area	Neuroscience,Transcription

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21000665	EK0861	96 wells/kit	$499		Add to Cart

Human PTX3/Pentraxin 3 ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human PTX3/Pentraxin 3 ELISA Kit PicoKine®

Rate it now..!!