Human PSG1 ELISA Kit PicoKine®

Mfr.No.

EK1997

Sensitivity

<50 pg/ml

Detection Range

1.56 ng/ml - 100 ng/ml

Reactivity

Human

Sample Volume

Concentration (ng/ml)	0	1.56	3.12	6.25	12.5	25	50	100
O.D.	0.013	0.082	0.164	0.313	0.466	0.796	1.403	2.156

Description

Human PSG1 ELISA Kit PicoKine® (96 Tests). Quantitate Human PSG1 in cell culture supeatants, serum, plasma (heparin or EDTA) and urine. Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Pregnancy-specific beta-1-glycoprotein 1; PS-beta-G-1; PSBG-1; Pregnancy-specific glycoprotein 1; CD66 antigen-like family member F; Fetal liver non-specific cross-reactive antigen 1/2; FL-NCA-1/2; PSG95; Pregnancy-specific beta-1 glycoprotein C/D; PS-beta-C/D; CD66f; PSG1; B1G1; PSBG; PSGGA

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q35-P419

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human PSG1

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma, Urine

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	2327	9714	48035	2915	11475	48927
Standard deviation	114.02	456.56	1969.44	163.24	585.23	3229.18
CV (%)	4.9%	4.7%	4.1%	5.6%	5.1%	6.6%

Background

The PSG1 is a semi-automatic sniper rifle? Pregnancy-specific beta-1 glycoprotein 1 (PSG-1) is also known as SP1, PSbG1, or B1G1, and is designated CD66f. It is mapped to 19q13.2. The human placenta is a multihormonal endocrine organ that produces hormones, enzymes, and other molecules that support fetal survival and development. Pregnancy-specific beta-1-glycoprotein (PSBG, PSG) is a major product of the syncytiotrophoblast, reaching concentrations of 100 to 290 mg/l at term in the serum of pregnant women. PSG is a member of the immunoglobulin (Ig) superfamily.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	2327	2279	2306	2344	2314	24.27	1.0%
Sample 2	9714	10138	10569	10990	10352	476.17	4.5%
Sample 3	48035	41237	47332	49070	46418	3054.74	6.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	5669
Gene Name	PSG1
UniProt ID	P11464

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001510	EK1997	96 wells/kit	$499		Add to Cart

Human PSG1 ELISA Kit PicoKine®

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