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Home / ELISA Kit

Human Protocadherin gamma-A2 (PCDHGA2) ELISA Kit

SKU : BHE13703511

Cadherins are calcium-dependent cell-cell adhesion molecules, and protocadherins constitute a subfamily of nonclassic cadherins. PCDHGA2 is 1 of 22 tandemly arranged genes within the PCDHG gene cluster on chromosome 5q31. The 22 PCDHG genes function as 'variable' exons that are individually spliced to a downstream constant region to form distinct PCDHG transcripts. The variable PCDHG exons encode the extracellular and transmembrane domains of the protocadherin protein, and the common region encodes the intracellular domain. For further information on the PCDHG.
By genomic sequence analysis, Wu et al. (2001) mapped the PCDHGA2 gene to 5q31, between the PCDHGA1 gene and the PCDHGA3 gene. They localized the mouse PCDHG genes to chromosome 18c.

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Mfr.No.	KTE61295
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Protocadherin gamma-A2 (PCDHGA2) ELISA Kit employs a two-site sandwich ELISA to quantitate PCDHGA2.
Alternative Names	PCDHGA2; PCDH-GAMMA-A2;
Detection Method	Colorimetric
Kit Components	Human Protocadherin gamma-A2 microplate Human Protocadherin gamma-A2 standard Human Protocadherin gamma-A2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Protocadherin gamma-A2 (PCDHGA2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PCDHGA2. No significant cross-reactivity or interference between Human PCDHGA2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Cadherins are calcium-dependent cell-cell adhesion molecules, and protocadherins constitute a subfamily of nonclassic cadherins. PCDHGA2 is 1 of 22 tandemly arranged genes within the PCDHG gene cluster on chromosome 5q31. The 22 PCDHG genes function as 'variable' exons that are individually spliced to a downstream constant region to form distinct PCDHG transcripts. The variable PCDHG exons encode the extracellular and transmembrane domains of the protocadherin protein, and the common region encodes the intracellular domain. For further information on the PCDHG. By genomic sequence analysis, Wu et al. (2001) mapped the PCDHGA2 gene to 5q31, between the PCDHGA1 gene and the PCDHGA3 gene. They localized the mouse PCDHG genes to chromosome 18c.
Notes	This Human Protocadherin gamma-A2 (PCDHGA2) ELISA Kit employs a two-site sandwich ELISA to quantitate PCDHGA2 in samples. An antibody specific for PCDHGA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCDHGA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCDHGA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCDHGA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	56113

Applications	ELISA
Applications Notes	This Human Protocadherin gamma-A2 (PCDHGA2) ELISA Kit employs a two-site sandwich ELISA to quantitate PCDHGA2 in samples. An antibody specific for PCDHGA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCDHGA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCDHGA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCDHGA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703511	KTE61295	48 T	$339		Add to Cart
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BHE13703511	KTE61295	96 Tx50	$24569		Add to Cart

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