Human Proto-oncogene serine/threonine-protein kinase pim-1(PIM1) ELISA kit

Human Proto-oncogene serine/threonine-protein kinase pim-1(PIM1) ELISA kit

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Mfr.No.

CSB-E11825h

Sensitivity

0.078 ng/mL

Detection Range

0.312 ng/mL-20 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

pim-1 oncogene, PIM, Oncogene PIM1, non-specific serine/threonine protein kinase, pim-1 kinase 44 kDa isoform, pim-1 oncogene (proviral integration site 1)

Recovery

The recovery of human ?GAL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	92	88-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ?GAL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	100
	Range %	95-110
1:10	Average %	97
	Range %	92-101
1:20	Average %	86
	Range %	82-90
1:40	Average %	93
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

µlU/ml	OD1	OD2	Average	Corrected
500	2.046	1.952	1.999	1.800
250	1.742	1.665	1.704	1.505
125	1.365	1.404	1.385	1.186
62.5	1.057	1.109	1.083	0.884
31.2	0.824	0.786	0.805	0.606
15.6	0.511	0.538	0.525	0.326
7.8	0.342	0.329	0.336	0.137
0	0.201	0.197	0.199

Species

Homo sapiens

Sample Type

Serum, Cell culture supernatants, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P11309
Tissue Specificity	Expressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 and isoform 2 are both expressed in prostate cancer cell lines.

Applications	ELISA
Research Area	PIM1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504060	CSB-E11825h	96T	$695	Add to Cart
BHE10504060	CSB-E11825h	5x96T	$3336	Add to Cart
BHE10504060	CSB-E11825h	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.