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Home / ELISA Kit

Human Proteoglycan 3 (PRG3) ELISA Kit

SKU : BHE13703382

Through large-scale sequencing, Plager et al. (1999) identified PRG3 as one of several transcripts upregulated in umbilical cord precursor cells following stimulation with interleukin-5. Using this sequence as probe, Plager et al. (2001) cloned PRG3 from a phage genomic library of placental tissues. Plager et al. (1999) found that the cDNA sequence predicts a mature 117-amino acid protein with a prepro- region of 108 amino acids. PRG3 shares 49% amino acid sequence identity with PRG2. RT-PCR demonstrated that PRG3 is expressed exclusively in bone marrow. Protein purified from eosinophil granules showed a molecular mass of 13.4 kD.Plager et al. (1999) found that the biologic activity of PRG3 is similar to that of PRG2 in cell killing and in neutrophil and basophil stimulation assays, but usually with reduced potency.

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Mfr.No.	KTE61166
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Proteoglycan 3 (PRG3) ELISA Kit employs a two-site sandwich ELISA to quantitate PRG3.
Alternative Names	PRG3; MBP2; MBPH; MGC126662; MGC141971; prepro-major basic protein homolog
Detection Method	Colorimetric
Kit Components	Human Proteoglycan 3 microplate Human Proteoglycan 3 standard Human Proteoglycan 3 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Proteoglycan 3 (PRG3) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRG3. No significant cross-reactivity or interference between Human PRG3 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Through large-scale sequencing, Plager et al. (1999) identified PRG3 as one of several transcripts upregulated in umbilical cord precursor cells following stimulation with interleukin-5. Using this sequence as probe, Plager et al. (2001) cloned PRG3 from a phage genomic library of placental tissues. Plager et al. (1999) found that the cDNA sequence predicts a mature 117-amino acid protein with a prepro- region of 108 amino acids. PRG3 shares 49% amino acid sequence identity with PRG2. RT-PCR demonstrated that PRG3 is expressed exclusively in bone marrow. Protein purified from eosinophil granules showed a molecular mass of 13.4 kD.Plager et al. (1999) found that the biologic activity of PRG3 is similar to that of PRG2 in cell killing and in neutrophil and basophil stimulation assays, but usually with reduced potency.
Notes	This Human Proteoglycan 3 (PRG3) ELISA Kit employs a two-site sandwich ELISA to quantitate PRG3 in samples. An antibody specific for PRG3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRG3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRG3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRG3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	10394

Applications	ELISA
Applications Notes	This Human Proteoglycan 3 (PRG3) ELISA Kit employs a two-site sandwich ELISA to quantitate PRG3 in samples. An antibody specific for PRG3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRG3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRG3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRG3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703382	KTE61166	48 T	$339		Add to Cart
BHE13703382	KTE61166	96 T	$589		Add to Cart
BHE13703382	KTE61166	96 Tx5	$2629		Add to Cart
BHE13703382	KTE61166	96 Tx50	$24569		Add to Cart

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