Human Protein Wnt-7B - WNT7B ELISA Kit

Mfr.No.

E2354Hu

Sensitivity

0.05ng/ml

Detection Range

0.1-30ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Wingless Type MMTV Integration Site Family, Member 7B (also known as WNT7B) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

Protein Wnt-7b;WNT7B

Molecular Weight

39327

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E2354Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2.66	0.22	8.27
Intra-Assay	2	18	4.2	0.29	7
Intra-Assay	3	18	10.21	0.36	3.56

Background

Ligand for members of the frizzled family of seven transmembrane receptors that functions in the canonical Wnt/beta-catenin signaling pathway (PubMed:30026314). Required for normal fusion of the chorion and the allantois during placenta development (By similarity). Required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation (PubMed:30026314).

Protocol

These standard curves of E2354Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
16ng/ml	2.21	2.152	2.1
	2.094
8ng/ml	1.371	1.255	1.203
	1.139
4ng/ml	0.74	0.782	0.73
	0.823
2ng/ml	0.516	0.487	0.435
	0.459
1ng/ml	0.178	0.222	0.17
	0.266
0ng/ml	0.053	0.052	0
	0.051

Gene ID	7477
UniProt ID	P56706

Research Area	Stem Cells

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103892	E2354Hu	48T	$320		Add to Cart
BHE12103892	E2354Hu	96T	$458		Add to Cart

Human Protein Wnt-7B - WNT7B ELISA Kit

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