Human Proteasome Activator Complex Subunit 1

Mfr.No.

E5948Hu

Sensitivity

8.95ng/L

Detection Range

20-3500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

11S regulator complex subunit alpha;Activator of multicatalytic protease subunit 1;HEL-S-129m;IFI5111;IGUP I-5111;Interferon gamma up-regulated I-5111 protein;PA28A;PA28alpha;Proteasome activator 28 subunit alpha;Proteasome activator complex subunit 1;PSME1;REGalpha;REG-alpha;

Molecular Weight

28723

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E5948Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	929.34	24.62	2.65
Intra-Assay	2	18	791.23	38.75	4.9
Intra-Assay	3	18	383.28	17.27	4.51

Background

Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.

Protocol

These standard curves of E5948Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2000ng/L	2.032	2.022	1.984
	2.013
1000ng/L	1.56	1.509	1.471
	1.458
500ng/L	0.677	0.612	0.575
	0.548
250ng/L	0.432	0.417	0.379
	0.402
125ng/L	0.146	0.164	0.126
	0.181
0ng/L	0.047	0.038	0
	0.029

Gene ID	5720
UniProt ID	Q06323

Research Area	Cell Biology; Microbiology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107310	E5948Hu	48T	$320		Add to Cart
BHE12107310	E5948Hu	96T	$458		Add to Cart

Human Proteasome Activator Complex Subunit 1 - PSME1 ELISA Kit

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