Human Prostate-specific antigen ELISA kit (PSA/KLK3) PicoKine®

Mfr.No.

EK0816

Sensitivity

<10 pg/ml

Detection Range

312 pg/ml - 20,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.005	0.118	0.224	0.378	0.702	1.346	1.694	2.078

Description

Human Kallikrein 3/PSA/KLK3 ELISA Kit PicoKine® (96 Tests). Quantitate Human KLK3 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Prostate-specific antigen;PSA;3.4.21.77;Gamma-seminoprotein;Seminin;Kallikrein-3;P-30 antigen;Semenogelase;KLK3;APS;

Molecular Weight

28741 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: A18-P261

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Kallikrein 3

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	551	2528	8207	568	2328	8686
Standard deviation	36.91	156.73	648.35	47.14	144.33	773.05
CV (%)	6.7%	6.2%	7.9%	8.3%	6.2%	8.9%

Protein Function

Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.

Background

Prostate-specific antigen (PSA), also known as kallikrein III, seminin, semenogelase, gamma-seminoprotein and P-30 antigen) is a 34 kD glycoprotein manufactured almost exclusively by the prostate gland; PSA is produced for the ejaculate where it liquifies the semen in the seminal coagulum and allows sperm to swim freely.1It is also believed to be instrumental in dissolving the cervical mucous cap, allowing the entry of sperm.2It is a serine protease enzyme, the gene of which is located on the nineteenth chromosome (19q13).3

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	551	505	496	595	536	39.57	7.3%
Sample 2	2528	2531	2745	2357	2540	137.6	5.4%
Sample 3	8207	9826	9283	9589	9226	619.14	6.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	354
Gene Name	KLK3
UniProt ID	P07288
Protein Name	Prostate-specific antigen
Sequence	Belongs to the peptidase S1 family. Kallikrein subfamily.

Applications	ELISA
Research Area	Cell Type Markers,Cytoskeleton/ECM,ECM Enzymes,Extracellular Matrix,Neuroscience,Signal Transduction,Tags & Cell Markers,Tumor Antigens,Tumor Associated,Tumor Biomarkers,Tumor Immunology,Tumor-Associated Antigens

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000633	EK0816	96 wells/kit	$499		Add to Cart

Human Prostate-specific antigen ELISA kit (PSA/KLK3) PicoKine®

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