Human Prostasin ELISA Kit PicoKine®

Mfr.No.

EK1272

Sensitivity

<75 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1,250	2,500	5,000	10,000
O.D.	0.019	0.329	0.558	0.785	0.990	1.266	1.516	1.825

Description

Human Prostasin ELISA Kit PicoKine® (96 Tests). Quantitate Human PRSS8 in cell culture supeatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 75 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Prostasin;3.4.21.-;Channel-activating protease 1;CAP1;Serine protease 8;Prostasin light chain;Prostasin heavy chain;PRSS8;

Molecular Weight

36431 MW

Immunogen

Expression system for standard: CHO; Immunogen sequence: A33-G319

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Prostasin

Subcellular Localization

Prostasin: Cell membrane; Single-pass membrane protein.

Sample Type

Cell culture supeatants, Serum, Plasma, Urine

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	267	577	3816	239	418	3890
Standard deviation	12.04	18.93	247.17	16.37	19.65	237.21
CV (%)	4.5%	3.3%	6.3%	6.9%	4.7%	6%

Protein Function

Possesses a trypsin-like cleavage specificity with a preference for poly-basic substrates. Stimulates epithelial sodium channel (ENaC) activity through activating cleavage of the gamma subunits (SCNN1G). .

Background

Prostasin is a protein that in humans is encoded by the PRSS8 gene. It is a single-copy gene and mapped to 16p11.2. Prostasin is likely to be the more physiologically relevant protease. This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. Coexpression of Prostasin with either Xenopus or rat ENaC in Xenopus oocytes resulted in a 60% to 80% increase in amiloride-sensitive sodium currents, and that the addition of aprotinin, a serine protease inhibitor, completely prevented this activation. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. The proprotein is cleaved to produce a light chain and a heavy chain which are associated by a disulfide bond. It is active on peptide linkages involving the carboxyl group of lysine or arginine.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	267	199	286	271	256	15.68	6.1%
Sample 2	362	319	570	544	449	18.71	4.2%
Sample 3	3489	4836	3635	4078	4010	198.87	5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	5652
Gene Name	PRSS8
UniProt ID	Q16651
Protein Name	Prostasin
Tissue Specificity	Found in prostate, liver, salivary gland, kidney, lung, pancreas, colon, bronchus and renal proximal tubular cells. In the prostate gland it may be synthesized in epithelial cells, secreted into the ducts, and excreted into the seminal fluid.
Sequence	Belongs to the peptidase S1 family.

Applications	ELISA
Research Area	Cell Biology,Proteolysis/Ubiquitin,Proteolytic Enzymes

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000862	EK1272	96 wells/kit	$499		Add to Cart

Human Prostasin ELISA Kit PicoKine®

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