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Home / ELISA Kit

Human Prostaglandin F2-alpha receptor (PTGFR) ELISA Kit

SKU : BHE13703287

Prostaglandin F2-alpha receptor encoded by PTGFR is member of the G-protein coupled receptor family. Prostaglandin F2-alpha receptor is a receptor for prostaglandin F2-alpha (PGF2-alpha), which is known to be a potent luteolytic agent, and may also be involved in modulating intraocular pressure and smooth muscle contraction in uterus. Knockout studies in mice suggest that the interaction of PGF2-alpha with this receptor may initiate parturition in ovarian luteal cells and thus induce luteolysis. Two transcript variants encoding different isoforms have been found for PTGFR.

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Mfr.No.	KTE61071
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Prostaglandin F2-alpha receptor (PTGFR) ELISA Kit employs a two-site sandwich ELISA to quantitate PTGFR.
Alternative Names	PTGFR; RP5-944H6.1; FP; MGC120498; MGC46203; OTTHUMP00000011219; PGF receptor; PGF2 alpha receptor; prostaglandin F receptor; prostaglandin F2 alpha receptor; prostaglandin receptor (2-alpha); prostanoid FP rece
Detection Method	Colorimetric
Kit Components	Human Prostaglandin F2-alpha receptor microplate Human Prostaglandin F2-alpha receptor standard Human Prostaglandin F2-alpha receptor detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Prostaglandin F2-alpha receptor (PTGFR) ELISA Kit has high sensitivity and excellent specificity for detection of Human PTGFR. No significant cross-reactivity or interference between Human PTGFR and analogues was observed.
Calibration Range	Please inquire
Sample Type	Serum, Cell culture supeatants, Plasma, Other biological fluids
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Prostaglandin F2-alpha receptor encoded by PTGFR is member of the G-protein coupled receptor family. Prostaglandin F2-alpha receptor is a receptor for prostaglandin F2-alpha (PGF2-alpha), which is known to be a potent luteolytic agent, and may also be involved in modulating intraocular pressure and smooth muscle contraction in uterus. Knockout studies in mice suggest that the interaction of PGF2-alpha with this receptor may initiate parturition in ovarian luteal cells and thus induce luteolysis. Two transcript variants encoding different isoforms have been found for PTGFR.
Notes	This Human Prostaglandin F2-alpha receptor (PTGFR) ELISA Kit employs a two-site sandwich ELISA to quantitate PTGFR in samples. An antibody specific for PTGFR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTGFR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTGFR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTGFR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	5737

Applications	ELISA
Applications Notes	This Human Prostaglandin F2-alpha receptor (PTGFR) ELISA Kit employs a two-site sandwich ELISA to quantitate PTGFR in samples. An antibody specific for PTGFR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTGFR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTGFR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTGFR bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703287	KTE61071	48 T	$339		Add to Cart
BHE13703287	KTE61071	96 T	$589		Add to Cart
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BHE13703287	KTE61071	96 Tx50	$24569		Add to Cart

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