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Home / ELISA Kit

Human Prospero homeobox protein 2 (PROX2) ELISA Kit

SKU : BHE13703336

PROX2 Belongs to the Prospero homeobox family.Contains 1 Prospero-type homeobox DNA-binding domain.Transcription regulator. Does not seem to be essential for embryonic development and postnatal survival.
The prospero domain coincides with a domain in a Drosophila gene that is expressed in Drosophila central nervous system and in the developing lens-secreting cone cells of the eye. Exon 1 of the human sequence showed no clear similarity to the 3 alternatively spliced variants of chicken PROX1 cDNA. Human PROX1 exon 1 was found to contain repetitive sequences. Analysis of mRNA revealed that Prox mRNA was present in many different human tissues and that lens demonstrated the highest level. In all tissues but lens, PROX1 cDNA hybridized to a single band of 8 kb.

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Mfr.No.	KTE61120
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Prospero homeobox protein 2 (PROX2) ELISA Kit employs a two-site sandwich ELISA to quantitate PROX2.
Alternative Names	PROX2; FLJ36749;
Detection Method	Colorimetric
Kit Components	Human Prospero homeobox protein 2 microplate Human Prospero homeobox protein 2 standard Human Prospero homeobox protein 2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Prospero homeobox protein 2 (PROX2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PROX2. No significant cross-reactivity or interference between Human PROX2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	PROX2 Belongs to the Prospero homeobox family.Contains 1 Prospero-type homeobox DNA-binding domain.Transcription regulator. Does not seem to be essential for embryonic development and postnatal survival. The prospero domain coincides with a domain in a Drosophila gene that is expressed in Drosophila central nervous system and in the developing lens-secreting cone cells of the eye. Exon 1 of the human sequence showed no clear similarity to the 3 alternatively spliced variants of chicken PROX1 cDNA. Human PROX1 exon 1 was found to contain repetitive sequences. Analysis of mRNA revealed that Prox mRNA was present in many different human tissues and that lens demonstrated the highest level. In all tissues but lens, PROX1 cDNA hybridized to a single band of 8 kb.
Notes	This Human Prospero homeobox protein 2 (PROX2) ELISA Kit employs a two-site sandwich ELISA to quantitate PROX2 in samples. An antibody specific for PROX2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPROX2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PROX2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PROX2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	283571

Applications	ELISA
Applications Notes	This Human Prospero homeobox protein 2 (PROX2) ELISA Kit employs a two-site sandwich ELISA to quantitate PROX2 in samples. An antibody specific for PROX2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPROX2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PROX2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PROX2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703336	KTE61120	48 T	$339		Add to Cart
BHE13703336	KTE61120	96 T	$589		Add to Cart
BHE13703336	KTE61120	96 Tx5	$2629		Add to Cart
BHE13703336	KTE61120	96 Tx50	$24569		Add to Cart

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