Human PROS1 / Protein S ELISA Kit PicoKine®

Mfr.No.

EK1739

Sensitivity

<50 pg/ml

Detection Range

312 pg/ml - 20,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.039	0.088	0.165	0.260	0.420	0.758	1.335	1.846

Description

Human PROS1 / Protein S ELISA Kit PicoKine® (96 Tests). Quantitate Human PROS1 in cell culture supeatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Vitamin K-dependent protein S; PROS1; PROS

Immunogen

Expression system for standard: NS0; Immunogen sequence: A42-S676

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human PROS1

Subcellular Localization

Secreted

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	497	2158	8049	521	2354	9179
Standard deviation	20.87	92.79	330.01	34.39	160.07	651.71
CV (%)	4.2%	4.3%	4.1%	6.6%	6.8%	7.1%

Protein Function

Anticoagulant plasma protein; it is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa. It helps to prevent coagulation and stimulating fibrinolysis.

Background

This PROS1 gene encodes a vitamin K-dependent plasma protein that functions as a cofactor for the anticoagulant protease, activated protein C (APC) to inhibit blood coagulation. This gene is mapped to 3q11.1. It is found in plasma in both a free, functionally active form and also in an inactive form complexed with C4bbinding protein. Mutations in this gene result in autosomal dominant hereditary thrombophilia. An inactive pseudogene of this locus is located at an adjacent region on chromosome 3. Alteative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	497	513	464	513	496	20.00	4.0%
Sample 2	2158	2442	2327	2374	2325	104.86	4.5%
Sample 3	8049	8437	8377	9288	8537	457.64	5.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	5627
Gene Name	PROS1
UniProt ID	P07225
Tissue Specificity	Plasma.

Applications	ELISA
Research Area	Cardiovascular,Serum Proteins

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001390	EK1739	96 wells/kit	$750		Add to Cart

Human PROS1 / Protein S ELISA Kit PicoKine®

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