Human Proprotein Convertase Subtilisin/Kexin Type 5

Mfr.No.

E2323Hu

Alternative Names

hPC6;PC5;PC6;PCSK 5;PCSK5;Proprotein convertase 5;Proprotein convertase 6;Proprotein convertase subtilisin/kexin type 5;Subtilisin/kexin-like protease PC5;

Reactivity

Human

Detection Range

10-2000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

206942

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

5.17ng/L

Background

Serine endoprotease that processes various proproteins by cleavage at paired basic amino acids, recognizing the RXXX(KR)R consensus motif. Likely functions in the constitutive and regulated secretory pathways. Plays an essential role in pregnancy establishment by proteolytic activation of a number of important factors such as BMP2, CALD1 and alpha-integrins.

Gene ID	5125
UniProt ID	Q92824
Antigen	PCSK5

Research Area

Signal Transduction

Protocol

These standard curves of E2323Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.079	2.221	2.172
	2.364
600ng/L	1.203	1.298	1.249
	1.392
300ng/L	0.724	0.683	0.634
	0.641
150ng/L	0.443	0.439	0.391
	0.435
75ng/L	0.21	0.19	0.141
	0.169
0ng/L	0.041	0.049	0
	0.057

Precision

We measured random samples of E2323Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	284.8	17.3	6.1
Intra-Assay	2	18	468.4	24.94	5.3
Intra-Assay	3	18	585.1	20.36	3.5

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103861	E2323Hu	48T	$320		Add to Cart
BHE12103861	E2323Hu	96T	$458		Add to Cart

Human Proprotein Convertase Subtilisin/Kexin Type 5 - PCSK5 ELISA Kit

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