Human Properdin / Complement Factor P ELISA Kit PicoKine®

Mfr.No.

EK1653

Sensitivity

<10 pg/ml

Detection Range

312 pg/ml - 20,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	312	625	200000000	20000000	2000000	200000	20000
O.D.	0.051	0.166	0.249	0.38	0.671	1.241	1.992	2.492

Description

Human Properdin / Complement Factor P ELISA Kit PicoKine® (96 Tests). Quantitate Human CFP in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Properdin; Complement factor P; CFP; PFC

Immunogen

Expression system for standard: NS0; Immunogen sequence: D28-L469

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Properdin

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	616	2125	9062	602	2285	9683
Standard deviation	28.33	91.37	561.84	30.1	109.68	629.39
CV (%)	4.6%	4.3%	6.2%	5%	4.8%	6.5%

Protein Function

A positive regulator of the alteate pathway of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes.

Background

Properdin or Factor P is the only known positive regulator of complement activation that stabilizes thealteative pathway convertases. This gene encodes a plasma glycoprotein that positively regulates the alteative complement pathway of the innate immune system. This protein binds to many microbial surfaces and apoptotic cells and stabilizes the C3- and C5-convertase enzyme complexes in a feedback loop that ultimately leads to formation of the membrane attack complex and lysis of the target cell. Mutations in this gene result in two forms of properdin deficiency, which results in high susceptibility to meningococcal infections. Multiple alteatively spliced variants, encoding the same protein, have been identified.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	616	576	554	549	573	26.42	4.6%
Sample 2	2125	2256	2211	2286	2219	60.73	2.7%
Sample 3	9062	9597	10032	10437	9782	510.91	5.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	5199
Gene Name	CFP
UniProt ID	P27918

Applications	ELISA
Research Area	Microbiology,Protein

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001321	EK1653	96 wells/kit	$499		Add to Cart

Human Properdin / Complement Factor P ELISA Kit PicoKine®

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