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Home / ELISA Kit

Human Proline dehydrogenase 1, mitochondrial (PRODH) ELISA Kit

SKU : BHE13703350

PRODH encodes a mitochondrial protein that catalyzes the first step in proline degradation. Mutations in PRODH are associated with hyperprolinemia type 1 and susceptibility to schizophrenia 4 (SCZD4). PRODH is located on chromosome 22q11.21, a region which has also been associated with the contiguous gene deletion syndromes, DiGeorge and CATCH22. Alternatively spliced transcript variants encoding different isoforms have been found for PRODH.

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Abbkine Scientific Co., Ltd.

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Mfr.No.	KTE61134
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Proline dehydrogenase 1, mitochondrial (PRODH) ELISA Kit employs a two-site sandwich ELISA to quantitate PRODH.
Alternative Names	PRODH; FLJ33744; HSPOX2; MGC148078; MGC148079; PIG6; POX; PRODH1; PRODH2; SCZD4; TP53I6; proline dehydrogenase 1; proline oxidase 2; proline oxidase; mitochondrial; tumor protein p53 inducible protein 6
Detection Method	Colorimetric
Kit Components	Human Proline dehydrogenase 1, mitochondrial microplate Human Proline dehydrogenase 1, mitochondrial standard Human Proline dehydrogenase 1, mitochondrial detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Proline dehydrogenase 1, mitochondrial (PRODH) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRODH. No significant cross-reactivity or interference between Human PRODH and analogues was observed.
Calibration Range	Please inquire
Sample Type	Serum, Cell culture supeatants, Plasma, Other biological fluids
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	PRODH encodes a mitochondrial protein that catalyzes the first step in proline degradation. Mutations in PRODH are associated with hyperprolinemia type 1 and susceptibility to schizophrenia 4 (SCZD4). PRODH is located on chromosome 22q11.21, a region which has also been associated with the contiguous gene deletion syndromes, DiGeorge and CATCH22. Alteatively spliced transcript variants encoding different isoforms have been found for PRODH.
Notes	This Human Proline dehydrogenase 1, mitochondrial (PRODH) ELISA Kit employs a two-site sandwich ELISA to quantitate PRODH in samples. An antibody specific for PRODH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRODH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRODH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRODH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	5625

Applications	ELISA
Applications Notes	This Human Proline dehydrogenase 1, mitochondrial (PRODH) ELISA Kit employs a two-site sandwich ELISA to quantitate PRODH in samples. An antibody specific for PRODH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRODH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRODH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRODH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703350	KTE61134	48 T	$339		Add to Cart
BHE13703350	KTE61134	96 T	$589		Add to Cart
BHE13703350	KTE61134	96 Tx5	$2629		Add to Cart
BHE13703350	KTE61134	96 Tx50	$24569		Add to Cart

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