Human Prolactin ELISA Kit PicoKine®

Mfr.No.

EK0593

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.027	0.123	0.217	0.409	0.780	1.237	1.553	1.933

Description

Human Prolactin ELISA Kit PicoKine® (96 Tests). Quantitate Human PRL in cell culture supeatants, serum and plasma (heparin, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Prolactin;PRL;PRL;

Molecular Weight

25876 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: L29-C227

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Prolactin

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	278	1989	4706	274	1928	5020
Standard deviation	18.07	95.47	338.83	21.64	129.17	371.48
CV (%)	6.5%	4.8%	7.2%	7.9%	6.7%	7.4%

Protein Function

Prolactin acts primarily on the mammary gland by promoting lactation.

Background

Prolactin, also calle PRL, is a protein that in humans is encodes by the PRL gene. This gene is mapped to 6p22.3. This gene encodes the anterior pituitary hormone prolactin. It may play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Prolactin also acts in a cytokine-like manner and as an important regulator of the immune system. Prolactin has important cell cycle related functions as a growth-, differentiating- and anti-apoptotic factor. As a growth factor binding to cytokine like receptors it has also profound influence on hematopoiesis,angiogenesis and is involved in the regulation of blood clotting through several pathways.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	278	266	323	299	291	21.68	7.4%
Sample 2	1989	1892	1944	1862	1921	48.66	2.5%
Sample 3	4706	4695	5058	4926	4846	153.08	3.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	5617
Gene Name	PRL
UniProt ID	P01236
Protein Name	Prolactin
Sequence	Belongs to the somatotropin/prolactin family.

Applications	ELISA
Research Area	Growth Factors/Hormones,Lipid Metabolism,Metabolism,Protein Phosphorylation,Receptor Tyrosine Kinases,Signal Transduction,Tyrosine Kinases

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21000538	EK0593	96 wells/kit	$499		Add to Cart

Human Prolactin ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human Prolactin ELISA Kit PicoKine®

Rate it now..!!