Human Progranulin ELISA Kit PicoKine®

Mfr.No.

EK0973

Sensitivity

<150 pg/ml

Detection Range

312 pg/ml - 20,000 pg/ml

Reactivity

Human

Sample Volume

Concentration(pg/ml)	0	312	625	1,250	2,500	5,000	10,000	20,000
O.D.	0.008	0.556	0.791	1.059	1.644	1.886	2.057	2.019

Description

Human Progranulin ELISA Kit PicoKine® (96 Tests). Quantitate Human GRN in cell culture supeatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. Sensitivity: 150pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Granulins;Proepithelin;PEPI;Acrogranin;Glycoprotein of 88 Kda;GP88;Glycoprotein 88;Progranulin;Paragranulin;Granulin-1;Granulin G;Granulin-2;Granulin F;Granulin-3;Granulin B;Granulin-4;Granulin A;Granulin-5;Granulin C;Granulin-6;Granulin D;Granulin-7;Granulin E;GRN;

Molecular Weight

63544 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: T18-L593

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Progranulin

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma, Saliva, Urine, Human milk

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	507	1173	9591	518	1169	9230
Standard deviation	36.35	71.9	321.3	16.37	88.96	699.63
CV(%)	7.2%	6.1%	3.4%	3.2%	7.6%	7.6%

Protein Function

Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling.

Background

Granulin, also known as Proepithelin (PEPI) is a protein that in humans is encoded by the GRN gene. This gene is mapped to 17q21.31. Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	507	529	566	377	495	28.05	5.7%
Sample 2	1173	1005	874	1096	1037	69.89	6.7%
Sample 3	9591	8789	9119	5790	8322	566.75	6.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	2896
Gene Name	GRN
UniProt ID	P28799
Protein Name	Granulins
Tissue Specificity	In myelogenous leukemic cell lines of promonocytic, promyelocytic, and proerythroid lineage, in fibroblasts, and very strongly in epithelial cell lines. Present in inflammatory cells and bone marrow. Highest levels in kidney.
Sequence	Belongs to the granulin family.

Applications	ELISA
Research Area	Cancer,Immunology,Neurodegenerative Disease,Neurology Process,Neuroscience,Secreted Molecules,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000736	EK0973	96 wells/kit	$499		Add to Cart

Human Progranulin ELISA Kit PicoKine®

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