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Home / ELISA Kit

Human Programmed cell death 1 ligand 2 (PDCD1LG2) ELISA Kit

SKU : BHE13703495

Using flow cytometric analysis, Latchman et al. (2001) showed that the IgV region of mouse Pdl2, like Pdl1, binds to human PDCD1 but not to CTLA4, CD28 , or ICOS. Stimulation of mouse T cells with anti-CD3 and Pdl2 or Pdl1 resulted in inhibition of proliferation and cytokine production, and Pdl2 could inhibit T-cell receptor -CD28 signals at low antigen concentrations. Latchman et al. (2001) proposed that blocking of the PDL-PDCD1 pathway may enhance antitumor immunity, whereas stimulating this pathway may help downregulate graft rejection and autoimmune and allergic reactions.
Latchman et al. (2001) mapped the PDL2 gene to chromosome 9p24.2, in close proximity to the PDL1 gene. Tseng et al. (2001) mapped the mouse Pdl2 gene to chromosome 19C2-C3.

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Mfr.No.	KTE61279
Description	This Human Programmed cell death 1 ligand 2 (PDCD1LG2) ELISA Kit employs a two-site sandwich ELISA to quantitate PDCD1LG2.
Alternative Names	PDCD1LG2; B7DC; Btdc; CD273; MGC142238; MGC142240; PD-L2; PDCD1L2; PDL2; bA574F11.2; PD-1-ligand 2; programmed death ligand 2
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Programmed cell death 1 ligand 2 microplate Human Programmed cell death 1 ligand 2 standard Human Programmed cell death 1 ligand 2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Programmed cell death 1 ligand 2 (PDCD1LG2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PDCD1LG2. No significant cross-reactivity or interference between Human PDCD1LG2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Using flow cytometric analysis, Latchman et al. (2001) showed that the IgV region of mouse Pdl2, like Pdl1, binds to human PDCD1 but not to CTLA4, CD28 , or ICOS. Stimulation of mouse T cells with anti-CD3 and Pdl2 or Pdl1 resulted in inhibition of proliferation and cytokine production, and Pdl2 could inhibit T-cell receptor -CD28 signals at low antigen concentrations. Latchman et al. (2001) proposed that blocking of the PDL-PDCD1 pathway may enhance antitumor immunity, whereas stimulating this pathway may help downregulate graft rejection and autoimmune and allergic reactions. Latchman et al. (2001) mapped the PDL2 gene to chromosome 9p24.2, in close proximity to the PDL1 gene. Tseng et al. (2001) mapped the mouse Pdl2 gene to chromosome 19C2-C3.
Notes	This Human Programmed cell death 1 ligand 2 (PDCD1LG2) ELISA Kit employs a two-site sandwich ELISA to quantitate PDCD1LG2 in samples. An antibody specific for PDCD1LG2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDCD1LG2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDCD1LG2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDCD1LG2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	80380
Antigen	PDCD1LG2
Tag	PDCD1LG2
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Programmed cell death 1 ligand 2 (PDCD1LG2) ELISA Kit employs a two-site sandwich ELISA to quantitate PDCD1LG2 in samples. An antibody specific for PDCD1LG2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDCD1LG2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDCD1LG2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDCD1LG2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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BHE13703495	KTE61279	48 T	$339		Add to Cart
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