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Home / ELISA Kit

Human Progesterone induced blocking factor (PIBF) ELISA Kit

SKU : BHE13703430

PIBF encodes a predicted hydrophilic 757-amino acid alpha-helical protein with an N-terminal signal sequence, a nuclear localization signal, a leucine zipper motif, a basic zipper sequence, a PEST sequence, an endoplasmic reticulum membrane retention signal, and several potential N-glycosylation and phosphorylation sites. Immunoblot analysis, immunocytochemistry, and fluorescence microscopy of insulin-treated mammary carcinoma cells detected expression of a 34-kD secreted protein in cytoplasmic granules. In untreated cells, nucleus-associated expression of the full-length 90-kD protein was detected.PIBF1 contains 758 amino acids. multiple PIBF1 transcripts expressed at highest levels in testis. Moderate expression was detected in spleen, thymus, prostate, ovary, small intestine, and colon.

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Mfr.No.	KTE61214
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Progesterone induced blocking factor (PIBF) ELISA Kit employs a two-site sandwich ELISA to quantitate PIBF1.
Alternative Names	PIBF1; C13orf24; KIAA1008; PIBF; RP11-505F3.1; progesterone-induced blocking factor 1
Detection Method	Colorimetric
Kit Components	Human Progesterone induced blocking factor microplate Human Progesterone induced blocking factor standard Human Progesterone induced blocking factor detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Progesterone induced blocking factor (PIBF) ELISA Kit has high sensitivity and excellent specificity for detection of Human PIBF1. No significant cross-reactivity or interference between Human PIBF1 and analogues was observed.
Calibration Range	Please inquire
Sample Type	Serum, Cell culture supernatants, Plasma, Other biological fluids
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	PIBF encodes a predicted hydrophilic 757-amino acid alpha-helical protein with an N-terminal signal sequence, a nuclear localization signal, a leucine zipper motif, a basic zipper sequence, a PEST sequence, an endoplasmic reticulum membrane retention signal, and several potential N-glycosylation and phosphorylation sites. Immunoblot analysis, immunocytochemistry, and fluorescence microscopy of insulin-treated mammary carcinoma cells detected expression of a 34-kD secreted protein in cytoplasmic granules. In untreated cells, nucleus-associated expression of the full-length 90-kD protein was detected.PIBF1 contains 758 amino acids. multiple PIBF1 transcripts expressed at highest levels in testis. Moderate expression was detected in spleen, thymus, prostate, ovary, small intestine, and colon.
Notes	This Human Progesterone induced blocking factor (PIBF) ELISA Kit employs a two-site sandwich ELISA to quantitate PIBF1 in samples. An antibody specific for PIBF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPIBF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIBF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIBF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	10464

Applications	ELISA
Applications Notes	This Human Progesterone induced blocking factor (PIBF) ELISA Kit employs a two-site sandwich ELISA to quantitate PIBF1 in samples. An antibody specific for PIBF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPIBF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIBF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIBF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703430	KTE61214	48 T	$359		Add to Cart
BHE13703430	KTE61214	96 T	$619		Add to Cart
BHE13703430	KTE61214	96 Tx5	$2809		Add to Cart
BHE13703430	KTE61214	96 Tx50	$26209		Add to Cart

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