Human Procollagen C Proteinase Enhancer 1

Mfr.No.

E4157hu

Sensitivity

0.09ng/ml

Detection Range

0.2-70ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Procollagen C Proteinase Enhancer 1 (also known as PCPE1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

PCOLCE;PCPE-1;Procollagen C-endopeptidase enhancer 1;Procollagen COOH-terminal proteinase enhancer 1;Procollagen C-proteinase enhancer 1;Type 1 procollagen C-proteinase enhancer protein;Type I procollagen COOH-terminal proteinase enhancer

Molecular Weight

47972

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E4157hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	4.4	0.33	7.5
Intra-Assay	2	18	22	1.32	6
Intra-Assay	3	18	10.8	0.79	7.3

Background

Binds to the C-terminal propeptide of type I procollagen and enhances procollagen C-proteinase activity. C-terminal processed part of PCPE (CT-PCPE) may have an metalloproteinase inhibitory activity.

Protocol

These standard curves of E4157hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
40ng/ml	2.233	2.214	2.17
	2.195
20ng/ml	1.55	1.599	1.554
	1.648
10ng/ml	1.064	1.096	1.051
	1.128
5ng/ml	0.698	0.676	0.632
	0.654
2.5ng/ml	0.439	0.425	0.381
	0.411
0ng/ml	0.05	0.045	0
	0.039

Gene ID	5118
UniProt ID	Q15113

Research Area	Others

Storage	2-8°C

Reference	Procollagen C-proteinase enhancer-1 and renal failure in multiple myeloma Journal: Int Urol Nephrol. 2022 Nov;54(11):3033-3038.

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105558	E4157hu	48T	$320		Add to Cart
BHE12105558	E4157hu	96T	$458		Add to Cart

Human Procollagen C Proteinase Enhancer 1 - PCPE1 ELISA Kit

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