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Home / ELISA Kit

Human Probable histone-lysine N-methyltransferase PRDM7 (PRDM7) ELISA Kit

SKU : BHE13703401

Progressive diversification of paralogs after gene expansion is essential to increase their functional specialization. However, mode and tempo of this divergence remain mostly unclear. PRDM7 underwent major structural rearrangements that decreased the number of encoded Zn-Fingers and modified gene splicing. Through internal duplication and activation of a non-canonical splice site (GC-AG), PRDM7 can acquire a novel intron.
PRDM7 is a transcription factor of the PR-domain protein family. It contains a PR-domain and multiple zinc finger motifs. Transcription factors of the PR-domain family are known to be involved in cell differentiation and tumorigenesis. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

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Mfr.No.	KTE61185
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Probable histone-lysine N-methyltransferase PRDM7 (PRDM7) ELISA Kit employs a two-site sandwich ELISA to quantitate PRDM7.
Alternative Names	PRDM7; MGC129525; PFM4; PR-domain family protein 4
Detection Method	Colorimetric
Kit Components	Human Probable histone-lysine N-methyltransferase PRDM7 microplate Human Probable histone-lysine N-methyltransferase PRDM7 standard Human Probable histone-lysine N-methyltransferase PRDM7 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Probable histone-lysine N-methyltransferase PRDM7 (PRDM7) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRDM7. No significant cross-reactivity or interference between Human PRDM7 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Progressive diversification of paralogs after gene expansion is essential to increase their functional specialization. However, mode and tempo of this divergence remain mostly unclear. PRDM7 underwent major structural rearrangements that decreased the number of encoded Zn-Fingers and modified gene splicing. Through internal duplication and activation of a non-canonical splice site (GC-AG), PRDM7 can acquire a novel intron. PRDM7 is a transcription factor of the PR-domain protein family. It contains a PR-domain and multiple zinc finger motifs. Transcription factors of the PR-domain family are known to be involved in cell differentiation and tumorigenesis. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Notes	This Human Probable histone-lysine N-methyltransferase PRDM7 (PRDM7) ELISA Kit employs a two-site sandwich ELISA to quantitate PRDM7 in samples. An antibody specific for PRDM7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRDM7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRDM7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRDM7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	11105

Applications	ELISA
Applications Notes	This Human Probable histone-lysine N-methyltransferase PRDM7 (PRDM7) ELISA Kit employs a two-site sandwich ELISA to quantitate PRDM7 in samples. An antibody specific for PRDM7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRDM7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRDM7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRDM7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703401	KTE61185	48 T	$339		Add to Cart
BHE13703401	KTE61185	96 T	$589		Add to Cart
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BHE13703401	KTE61185	96 Tx50	$24569		Add to Cart

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