Human Probable E3 Ubiquitin-protein Ligase Makorin-3

Mfr.No.

E6435Hu

Description

This sandwich kit is for the accurate quantitative detection of human Probable E3 Ubiquitin-protein Ligase Makorin-3 (also known as MKRN3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CPPB2;D15S9;MKRN3;Probable E3 ubiquitin-protein ligase makorin-3;RING finger protein 63;RING-type E3 ubiquitin transferase makorin-3;RNF63;ZFP127;Zinc finger protein 127;ZNF127

Reactivity

Human

Detection Range

0.05-10ng/ml

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

55645

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.019ng/ml

Background

E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins.

Gene ID	7681
UniProt ID	Q13064
Antigen	MKRN3

Research Area

Others

Protocol

These standard curves of E6435Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
6.4ng/ml	1.94	1.869	1.831
	1.798
3.2ng/ml	1.02	1.032	0.994
	1.045
1.6ng/ml	0.553	0.531	0.493
	0.509
0.8ng/ml	0.21	0.18	0.142
	0.15
0.4ng/ml	0.101	0.082	0.044
	0.063
0ng/ml	0.037	0.038	0
	0.039

Precision

We measured random samples of E6435Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3.2	0.15	4.63
Intra-Assay	2	18	4.52	0.11	2.39
Intra-Assay	3	18	1.28	0.05	3.65

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107797	E6435Hu	48T	$320		Add to Cart
BHE12107797	E6435Hu	96T	$458		Add to Cart

Human Probable E3 Ubiquitin-protein Ligase Makorin-3 - MKRN3 ELISA Kit

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